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er α sequence  (Addgene inc)


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    Addgene inc er α sequence
    Er α Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/er α sequence/product/Addgene inc
    Average 93 stars, based on 46 article reviews
    er α sequence - by Bioz Stars, 2026-05
    93/100 stars

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    MyoD lentiviral delivery system. WT MyoD, VP64 MyoD, VP16 MyoD, and p65 MyoD were cloned into a Tet-ON lentiviral vector. This vector constitutively expresses the reverse tetracycline transactivator (rtTA2 S -M2) and the puromycin resistance gene (Puro R ) from the human phosphoglycerate kinase (hPGK) promoter. The rtTA2 S -M2 and Puro R are coexpressed from the same mRNA via an internal ribosomal entry site (IRES). The MyoD-T2A-dsRed Express2 expression cassette is downstream of the tetracycline response element (TRE) promoter. The rtTA2 S -M2 binds to the TRE and activates expression of the downstream genes in the presence of doxycycline. The T2A ribosomal skipping peptide results in the expression of two separate proteins from a single mRNA. The peptide sequence of each transcriptional activation domain is shown. The minimal activation domain of VP16 is shown in red. PKKKRKV is the SV40 nuclear localization signal and p65 contains the native nuclear localization signals KRKR and LGALL (underlined). VP64 also contains an HA epitope tag (YPYDVPDYA) that was not utilized in this study (underlined). All fusion proteins contain a flexible serine/glycine linker GGSGGGS (bold) between the activation domain and MyoD.

    Journal: ACS Synthetic Biology

    Article Title: Enhanced MyoD-Induced Transdifferentiation to a Myogenic Lineage by Fusion to a Potent Transactivation Domain

    doi: 10.1021/sb500322u

    Figure Lengend Snippet: MyoD lentiviral delivery system. WT MyoD, VP64 MyoD, VP16 MyoD, and p65 MyoD were cloned into a Tet-ON lentiviral vector. This vector constitutively expresses the reverse tetracycline transactivator (rtTA2 S -M2) and the puromycin resistance gene (Puro R ) from the human phosphoglycerate kinase (hPGK) promoter. The rtTA2 S -M2 and Puro R are coexpressed from the same mRNA via an internal ribosomal entry site (IRES). The MyoD-T2A-dsRed Express2 expression cassette is downstream of the tetracycline response element (TRE) promoter. The rtTA2 S -M2 binds to the TRE and activates expression of the downstream genes in the presence of doxycycline. The T2A ribosomal skipping peptide results in the expression of two separate proteins from a single mRNA. The peptide sequence of each transcriptional activation domain is shown. The minimal activation domain of VP16 is shown in red. PKKKRKV is the SV40 nuclear localization signal and p65 contains the native nuclear localization signals KRKR and LGALL (underlined). VP64 also contains an HA epitope tag (YPYDVPDYA) that was not utilized in this study (underlined). All fusion proteins contain a flexible serine/glycine linker GGSGGGS (bold) between the activation domain and MyoD.

    Article Snippet: The VP16 sequence was isolated from Addgene plasmid 11351.

    Techniques: Clone Assay, Plasmid Preparation, Expressing, Sequencing, Activation Assay